Apoptosis‐inducing factor deficiency sensitizes dopaminergic neurons to parkinsonian neurotoxins
Identifieur interne : 000812 ( Main/Exploration ); précédent : 000811; suivant : 000813Apoptosis‐inducing factor deficiency sensitizes dopaminergic neurons to parkinsonian neurotoxins
Auteurs : Celine Perier [Espagne] ; Jordi Bové [Espagne] ; Benjamin Dehay [Espagne] ; Vernice Jackson-Lewis [États-Unis] ; Peter S. Rabinovitch [États-Unis] ; Serge Przedborski [États-Unis] ; Miquel Vila [Espagne]Source :
- Annals of Neurology [ 0364-5134 ] ; 2010-08.
Abstract
Objective: Mitochondrial complex I deficits have long been associated with Parkinson disease (PD). However, it remains unknown whether such defects represent a primary event in dopaminergic neurodegeneration. Methods: Apoptosis‐inducing factor (AIF) is a mitochondrial protein that, independently of its proapoptotic properties, plays an essential physiologic role in maintaining a fully functional complex I. We used AIF‐deficient harlequin (Hq) mice, which exhibit structural deficits in assembled complex I, to determine whether primary complex I defects linked to AIF depletion may cause dopaminergic neurodegeneration. Results: Despite marked reductions in mitochondrial complex I protein levels, Hq mice did not display apparent alterations in the dopaminergic nigrostriatal system. However, these animals were much more susceptible to exogenous parkinsonian complex I inhibitors, such as 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP). Subtoxic doses of MPTP, unable to cause damage to wild‐type animals, produced marked nigrostriatal dopaminergic degeneration in Hq mice. This effect was associated with exacerbated complex I inhibition and increased production of mitochondrial‐derived reactive oxygen species (ROS) in Hq brain mitochondria. The antioxidant superoxide dismutase‐mimetic compound tempol was able to reverse the increased susceptibility of Hq mice to MPTP. Supporting an instrumental role for mitochondrial‐derived ROS in PD‐related neurodegeneration, transgenic mice overexpressing mitochondrially targeted catalase exhibited an attenuation of MPTP‐induced mitochondrial ROS and dopaminergic cell death. Interpretation: Structural complex I alterations linked to AIF deficiency do not cause dopaminergic neurodegeneration but increase the susceptibility of dopaminergic neurons to exogenous parkinsonian neurotoxins, reinforcing the concept that genetic and environmental factors may interact in a common molecular pathway to trigger PD. ANN NEUROL 2010;68:184–192
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DOI: 10.1002/ana.22034
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However, it remains unknown whether such defects represent a primary event in dopaminergic neurodegeneration. Methods: Apoptosis‐inducing factor (AIF) is a mitochondrial protein that, independently of its proapoptotic properties, plays an essential physiologic role in maintaining a fully functional complex I. We used AIF‐deficient harlequin (Hq) mice, which exhibit structural deficits in assembled complex I, to determine whether primary complex I defects linked to AIF depletion may cause dopaminergic neurodegeneration. Results: Despite marked reductions in mitochondrial complex I protein levels, Hq mice did not display apparent alterations in the dopaminergic nigrostriatal system. However, these animals were much more susceptible to exogenous parkinsonian complex I inhibitors, such as 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP). Subtoxic doses of MPTP, unable to cause damage to wild‐type animals, produced marked nigrostriatal dopaminergic degeneration in Hq mice. This effect was associated with exacerbated complex I inhibition and increased production of mitochondrial‐derived reactive oxygen species (ROS) in Hq brain mitochondria. The antioxidant superoxide dismutase‐mimetic compound tempol was able to reverse the increased susceptibility of Hq mice to MPTP. Supporting an instrumental role for mitochondrial‐derived ROS in PD‐related neurodegeneration, transgenic mice overexpressing mitochondrially targeted catalase exhibited an attenuation of MPTP‐induced mitochondrial ROS and dopaminergic cell death. Interpretation: Structural complex I alterations linked to AIF deficiency do not cause dopaminergic neurodegeneration but increase the susceptibility of dopaminergic neurons to exogenous parkinsonian neurotoxins, reinforcing the concept that genetic and environmental factors may interact in a common molecular pathway to trigger PD. ANN NEUROL 2010;68:184–192</div></front></TEI><affiliations><list><country><li>Espagne</li><li>États-Unis</li></country><region><li>Catalogne</li><li>Washington (État)</li><li>État de New York</li></region><settlement><li>Barcelone</li></settlement></list><tree><country name="Espagne"><region name="Catalogne"><name sortKey="Perier, Celine" sort="Perier, Celine" uniqKey="Perier C" first="Celine" last="Perier">Celine Perier</name></region><name sortKey="Bove, Jordi" sort="Bove, Jordi" uniqKey="Bove J" first="Jordi" last="Bové">Jordi Bové</name><name sortKey="Dehay, Benjamin" sort="Dehay, Benjamin" uniqKey="Dehay B" first="Benjamin" last="Dehay">Benjamin Dehay</name><name sortKey="Vila, Miquel" sort="Vila, Miquel" uniqKey="Vila M" first="Miquel" last="Vila">Miquel Vila</name><name sortKey="Vila, Miquel" sort="Vila, Miquel" uniqKey="Vila M" first="Miquel" last="Vila">Miquel Vila</name></country><country name="États-Unis"><region name="État de New York"><name sortKey="Jackson Ewis, Vernice" sort="Jackson Ewis, Vernice" uniqKey="Jackson Ewis V" first="Vernice" last="Jackson-Lewis">Vernice Jackson-Lewis</name></region><name sortKey="Przedborski, Serge" sort="Przedborski, Serge" uniqKey="Przedborski S" first="Serge" last="Przedborski">Serge Przedborski</name><name sortKey="Przedborski, Serge" sort="Przedborski, Serge" uniqKey="Przedborski S" first="Serge" last="Przedborski">Serge Przedborski</name><name sortKey="Przedborski, Serge" sort="Przedborski, Serge" uniqKey="Przedborski S" first="Serge" last="Przedborski">Serge Przedborski</name><name sortKey="Przedborski, Serge" sort="Przedborski, Serge" uniqKey="Przedborski S" first="Serge" last="Przedborski">Serge Przedborski</name><name sortKey="Rabinovitch, Peter S" sort="Rabinovitch, Peter S" uniqKey="Rabinovitch P" first="Peter S." last="Rabinovitch">Peter S. 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